A new coupon design for simultaneous analysis of in situ microbial biofilm formation and community structure in drinking water distribution systems

Deines, Peter, Sekar, Raju, Husband, P. Stewart , Boxall, Joby B., Osborn, A. Mark and Biggs, Catherine A. (2010) A new coupon design for simultaneous analysis of in situ microbial biofilm formation and community structure in drinking water distribution systems. Applied Microbiology and Biotechnology, 87 (2). pp. 749-756. ISSN 0175-7598

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This study presents a new coupon sampling device that can be inserted directly into the pipes within water distribution systems (WDS), maintaining representative near wall pipe flow conditions and enabling simultaneous microscopy and DNA-based analysis of biofilms formed in situ. To evaluate this sampling device, fluorescent in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) analyses were used to investigate changes in biofilms on replicate coupons within a non-sterile pilot-scale WDS. FISH analysis demonstrated increases in bacterial biofilm coverage of the coupon surface over time, while the DGGE analysis showed the development of increasingly complex biofilm communities, with time-specific clustering of these communities. This coupon design offers improvements over existing biofilm sampling devices in that it enables simultaneous quantitative and qualitative compositional characterization of biofilm assemblages formed within a WDS, while importantly maintaining fully representative near wall pipe flow conditions. Hence, it provides a practical approach that can be used to capture the interactions between biofilm formation and changing abiotic conditions, boundary shear stress, and turbulent driven exchange within WDS. © Springer-Verlag 2010.

Keywords:Abiotic conditions, Bacterial biofilm, Biofilm formation, Boundary shear stress, Community structures, Compositional characterization, Denaturing gradient gel electrophoresis, DGGE, Drinking water distribution system, Drinking water distribution systems, Fish analysis, Flow condition, Fluorescent in situ hybridization, In-situ, Microbial biofilm, Near-wall, Pilot scale, Sampling devices, Simultaneous analysis, Biofilms, Electrophoresis, Fish, Gels, Pipe, Pipe flow, Potable water, Water distribution systems, Water supply, Biofilters, 4',6 diamidino 2 phenylindole, drinking water, biofilm, community structure, detection method, drinking water, electrokinesis, genetic analysis, microbial activity, microbial community, shear stress, water supply, article, biofilm, fluorescence in situ hybridization, qualitative analysis, water sampling, water supply, Bacteria, Bacterial Physiological Phenomena, Biofilms, DNA, Bacterial, Equipment Design, Genetic Techniques, Microscopy, Water Microbiology, Water Supply, Bacteria (microorganisms)
Subjects:C Biological Sciences > C180 Ecology
C Biological Sciences > C500 Microbiology
Divisions:College of Science > School of Life Sciences
ID Code:8944
Deposited On:22 Apr 2013 08:01

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