Biological samples micro-manipulation by means of optical tweezers

Ferrari, Enrico, Emiliani, Valentina, Cojoc, Dan , Garbin, Valeria, Zahid, Morad, Durieux, Christiane, Coppey-Moisan, Maite and Di Fabrizio, Enzo (2005) Biological samples micro-manipulation by means of optical tweezers. Microelectronic Engineering, 78-79 . pp. 575-581. ISSN 0167-9317

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The goal of this work is to investigate the usefulness of the optical tweezers for biological sample micro-manipulation.

We report multiple optical trapping and manipulation of Escherichia coli cells, immersed in growth medium, by means of diffractive optical elements (DOE). The DOEs are implemented on a spatial light modulator to generate movable geometries of traps.

We report also an experiment that allows to mimic the mechanical environment of cells in tissues. Micro-meter sized beads are trapped in circular geometry in order to surround living cells. By dynamically varying the geometry of the configuration and the trapping forces, we can reproduce a controlled environment where only mechanical stimuli are present and biological responses can be monitored. These experiments have been implemented in two different setups. In the first, cell manipulation is performed by the use of two-dimensional optical tweezers system generated by dual axis acousto-optical deflectors. In the second setup, trapping configuration is extended to three-dimensional volumes using DOEs.

Additional Information:Proceedings of the 30th International Conference on Micro- and Nano-Engineering Rotterdam,Netherlands 19–22 September 2004
Keywords:Optical tweezers, Optical manipulation, Spatial light modulators
Subjects:F Physical Sciences > F360 Optical Physics
F Physical Sciences > F310 Applied Physics
F Physical Sciences > F361 Laser Physics
Divisions:College of Science > School of Life Sciences
ID Code:8386
Deposited On:29 Mar 2013 19:45

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