Learning impairment and cholinergic deafferentation after cortical nerve growth factor deprivation

Gutierrez, Humberto, Miranda, Maria Isabel and Bermúdez-Rattoni, Federico (1997) Learning impairment and cholinergic deafferentation after cortical nerve growth factor deprivation. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 17 (10). pp. 3796-3803. ISSN 1529-2401

Full content URL: http://www.jneurosci.org/content/17/10/3796.full

Full text not available from this repository.

Item Type:Article
Item Status:Live Archive


Cholinergic basal forebrain (CBF ) neurons have been shown to respond in vivo to exogenous administration of NGF. Although neurotrophins and their receptors are widely expressed in the CNS, little data exist for the physiological significance of endogenous neurotrophin signaling in CNS neurons. To test directly whether cortically derived NGF is functionally required for the cholinergic functions mediated by the cerebral cortex, repeated injections of anti-NGF mAbs were locally applied into the insular cortex (IC) of rats. The biochemical results, using an in vivo microdialysis technique, showed a dramatic lack of extracellular release of acetylcholine after high potassium stimulation compared with controls. Furthermore, by using small injections of the neurotracer fluorogold, we found a corresponding disruption in the connectivity between the IC and the CBF. Behavioral experiments showed that the NGF antibodies applied into the IC produced a significant disruption on the acquisition of conditioned taste aversion and inhibitory avoidance learning. However, the same animals were able to recall the taste aversion when the conditioning trial was established before injections of NGF antibodies. Given these results, it seems that cortical cholinergic functions are actively dependent on locally derived NGF in the adult normal brain, and that the cholinergic activity from the CBF is not necessary for recalling aversive stimuli, but is necessary for the acquisition of aversively motivated conditionings.

Keywords:Learning and memory
Subjects:C Biological Sciences > C120 Behavioural Biology
Divisions:College of Science > School of Life Sciences
ID Code:7864
Deposited On:21 Mar 2013 11:38

Repository Staff Only: item control page