Scanning x-ray microdiffraction of optically manipulated liposomes

Cojoc, D., Ferrari, Enrico, Garbin, V. , Di Fabrizio, E., Amenitsch, H., Rappolt, M., Sartori, B., Laggner, P., Burghammer, M. and Riekel, C. (2007) Scanning x-ray microdiffraction of optically manipulated liposomes. Applied Physics Letters, 91 (23). p. 234107. ISSN 0003-6951

Full content URL: http://apl.aip.org/resource/1/applab/v91/i23/p2341...

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Item Type:Article
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Abstract

We show optical tweezers manipulation of individual micron-sized samples investigating at the same time their inner nanostructure by synchrotron diffraction experiments. The validity of this technique is demonstrated for clusters of multilamellar liposomes trapped in single and multiple positions in the optical path of a microfocused x-ray beam and analyzed in a microscanning mode. The signal to background ratio of the first order peak shows that single liposome measurements are feasible. Multiple trapping by means of diffractive optical elements is demonstrated as an effective manipulation tool for future x-ray diffraction studies of the interaction between different sample entities.

Additional Information:We show optical tweezers manipulation of individual micron-sized samples investigating at the same time their inner nanostructure by synchrotron diffraction experiments. The validity of this technique is demonstrated for clusters of multilamellar liposomes trapped in single and multiple positions in the optical path of a microfocused x-ray beam and analyzed in a microscanning mode. The signal to background ratio of the first order peak shows that single liposome measurements are feasible. Multiple trapping by means of diffractive optical elements is demonstrated as an effective manipulation tool for future x-ray diffraction studies of the interaction between different sample entities.
Keywords:biological techniques, biomembranes, cellular biophysics, diffractive optical elements, radiation pressure, synchrotron radiation, X-ray diffraction
Subjects:F Physical Sciences > F310 Applied Physics
F Physical Sciences > F361 Laser Physics
Divisions:College of Science > School of Life Sciences
ID Code:6980
Deposited On:27 Nov 2012 23:21

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