Martinez-Fleites, Carlos, Tarbouriech, Nicolas, Ortiz-Lombardia, Miguel et al, Taylor, Edward, Rodriguez, Armando, Ramirez, Ricardo, Hernandez, Lazaro and Davies, Gideon J.
(2004)
Crystallization and preliminary X-ray diffraction analysis of levansucrase (LsdA) from Gluconacetobacter diazotrophicus SRT4.
Acta Crystallographica Section D, 60
(1).
pp. 181-183.
ISSN 0907-4449
Full content URL: http://dx.doi.org/10.1107/S0907444903025514
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| Item Type: | Article |
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| Item Status: | Live Archive |
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Abstract
The endophytic bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA; EC 2.4.1.10), which converts sucrose to fructo-oligosaccharides and levan. Fully active LsdA was purified to high homogeneity by non-denaturing reversed-phase HPLC and was crystallized at room temperature by the hanging-drop vapour-diffusion method using ammonium sulfate and ethanol as precipitants. The crystals are extremely sensitive, but native data have been collected to 2.5 A under cryogenic conditions using synchrotron radiation. LsdA crystals belong to the orthorhombic space group P22(1)2(1) or P2(1)2(1)2, with unit-cell parameters a = 53.80, b = 119.39, c = 215.10 A.
| Additional Information: | The endophytic bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA; EC 2.4.1.10), which converts sucrose to fructo-oligosaccharides and levan. Fully active LsdA was purified to high homogeneity by non-denaturing reversed-phase HPLC and was crystallized at room temperature by the hanging-drop vapour-diffusion method using ammonium sulfate and ethanol as precipitants. The crystals are extremely sensitive, but native data have been collected to 2.5 A under cryogenic conditions using synchrotron radiation. LsdA crystals belong to the orthorhombic space group P22(1)2(1) or P2(1)2(1)2, with unit-cell parameters a = 53.80, b = 119.39, c = 215.10 A. |
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| Keywords: | levansucrase, structural biology |
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| Subjects: | C Biological Sciences > C700 Molecular Biology, Biophysics and Biochemistry |
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| Divisions: | College of Science > School of Life Sciences |
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| ID Code: | 6178 |
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| Deposited On: | 20 Sep 2012 16:40 |
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