Protection of respiratory chain enzymes from ischaemic damage in adult rat brain slices

Brooks, Keith J., Hargreaves, Iain P. and Bates, Timothy E. (2008) Protection of respiratory chain enzymes from ischaemic damage in adult rat brain slices. Neurochemical research, 33 (9). pp. 1711-1716. ISSN 0364-3190

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The effect of aglycaemic hypoxia (AH) on the activity of the mitochondrial respiratory chain complexes was measured in superfused adult cortical brain slices. After 15 min of AH the activity of complex II-III was significantly reduced (by 45%) with no change in complex I or IV. Following 30 min of reperfusion the activities of complex II-III and IV were significantly reduced (by 45% and 20% respectively). These reductions in enzyme activity were abolished by removing the external calcium or by the addition of N omega-nitro-L-arginine (LNNA) or an analogue of superoxide dismutase (SOD) manganese [III] tetrakis 4-benzoic acid porphyrin (Mn-TBAP). These data suggest that a reactive oxygen species (ROS) such as peroxynitrite is involved in the reduction of mitochondrial complex activities following AH.

Keywords:Brain Slices, Ischaemia, Ischemia, Mitochondria, respiratory chain complexes, N omega-nitro-L-arginine, superoxide dismutase, manganese [III] tetrakis 4-benzoic acid porphyrin, peroxynitrite
Subjects:A Medicine and Dentistry > A100 Pre-clinical Medicine
B Subjects allied to Medicine > B200 Pharmacology, Toxicology and Pharmacy
B Subjects allied to Medicine > B140 Neuroscience
Divisions:College of Science > School of Life Sciences
ID Code:5201
Deposited On:26 Jun 2012 06:32

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