Luo, Xiaoqin, Ng, Choaping, He, Jingjing , Yang, Mengliu, Luo, Xiao, Herbert, Terence P. and Whitehead, Jon (2022) Vitamin C protects against hypoxia, inflammation, and ER stress in primary human preadipocytes and adipocytes. Molecular and Cellular Endocrinology, 556 (111740). pp. 1-10. ISSN 0303-7207
Full content URL: https://doi.org/10.1016/j.mce.2022.111740
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1-s2.0-S0303720722001885-main.pdf - Whole Document Available under License Creative Commons Attribution 4.0 International. 7MB |
Item Type: | Article |
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Item Status: | Live Archive |
Abstract
Dysregulation of adipose tissue involves increased cellular hypoxia, ER stress, and inflammation and altered adipokine production, contributing to the aetiology of obesity-related diseases including type 2 diabetes and cardiovascular disease. This study aimed to investigate the effects of Vitamin C supplementation on these processes in primary human preadipocytes and adipocytes. Treatment of preadipocytes and adipocytes with the proinflammatory cytokine TNFalpha and palmitic acid (PA), to mimic the obesogenic milieu, significantly increased markers of hypoxia, ER stress and inflammation and reduced secretion of high molecular weight (HMW) adiponectin. Importantly, Vitamin C abolished TNFalpha+PA induced hypoxia and significantly reduced the increases in ER stress and inflammation in both cell types. Vitamin C also significantly increased the secretion of HMW adiponectin from adipocytes. These findings indicate that Vitamin C can reduce obesity-associated cellular stress and thus provide a rationale for future investigations.
Keywords: | Ascorbic acid, Cytokines, Adiponectin, Obesity |
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Subjects: | A Medicine and Dentistry > A100 Pre-clinical Medicine |
Divisions: | College of Science > School of Life and Environmental Sciences > Department of Life Sciences |
ID Code: | 51941 |
Deposited On: | 03 Oct 2022 11:48 |
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