Shuaib, Yassir A, Utpatel, Christian, Kohl, Thomas A , Barilar, Ivan, Diricks, Margo, Ashraf, Nadia, Wieler, Lothar H, Kerubo, Glennah, Mesfin, Eyob A, Diallo, Awa Ba, Al-Hajoj, Sahal, Ndung'u, Perpetua, Fitzgibbon, Margaret M, Vaziri, Farzam, Sintchenko, Vitali, Martinez, Elena, Viegas, Sofia O, Zhou, Yang, Azmy, Aya, Al-Amry, Khaled, Godreuil, Sylvain, Varma-Basil, Mandira, Narang, Anshika, Ali, Solomon, Beckert, Patrick, Dreyer, Viola, Kabwe, Mwila, Bates, Matthew, Hoelscher, Michael, Rachow, Andrea, Gori, Andrea, Tekwu, Emmanuel M, Sidze, Larissa K, Jean-Paul, Assam A, Beng, Veronique P, Ntoumi, Francine, Frank, Matthias, Diallo, Aissatou Gaye, Mboup, Souleymane, Tessema, Belay, Beyene, Dereje, Khan, Sadiq N, Diel, Roland, Supply, Philip, Maurer, Florian P, Hoffmann, Harald, Niemann, Stefan and Merker, Matthias (2022) Origin and Global Expansion of Complex Lineage 3. Genes, 13 (6). ISSN 2073-4425
Full content URL: https://doi.org/10.3390/genes13060990
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Shuaib TB mol epi Genes 2022.pdf - Whole Document Available under License Creative Commons Attribution 4.0 International. 2MB |
| Item Type: | Article |
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| Item Status: | Live Archive |
Abstract
complex (MTBC) Lineage 3 (L3) strains are abundant in world regions with the highest tuberculosis burden. To investigate the population structure and the global diversity of this major lineage, we analyzed a dataset comprising 2682 L3 strains from 38 countries over 5 continents, by employing 24-loci mycobacterial interspersed repetitive unit-variable number of tandem repeats genotyping (MIRU-VNTR) and drug susceptibility testing. We further combined whole-genome sequencing (WGS) and phylogeographic analysis for 373 strains representing the global L3 genetic diversity. Ancestral state reconstruction confirmed that the origin of L3 strains is located in Southern Asia and further revealed multiple independent introduction events into North-East and East Africa. This study provides a systematic understanding of the global diversity of L3 strains and reports phylogenetic variations that could inform clinical trials which evaluate the effectivity of new drugs/regimens or vaccine candidates.
| Keywords: | Tuberculosis, Epidemiology, Genotype, DNA sequencing, Genomics, Microbial diversity |
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| Subjects: | C Biological Sciences > C500 Microbiology |
| Divisions: | College of Science > School of Life Sciences |
| Related URLs: | |
| ID Code: | 50079 |
| Deposited On: | 11 Jul 2022 08:06 |
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