Myosin VI regulates the spatial organisation of mammalian transcription initiation.

Hari-Gupta, Yukti, Fili, Natalia, Dos Santos, Ália , Cook, Alexander W, Gough, Rosemarie E, Reed, Hannah C W, Wang, Lin, Aaron, Jesse, Venit, Tomas, Wait, Eric, Grosse-Berkenbusch, Andreas, Gebhardt, J Christof M, Percipalle, Piergiorgio, Chew, Teng-Leong, Martin-Fernandez, Marisa and Toseland, Christopher P (2022) Myosin VI regulates the spatial organisation of mammalian transcription initiation. Nature Communications, 13 (1). p. 1346. ISSN 2041-1723

Full content URL: https://doi.org/10.1038/s41467-022-28962-w

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Myosin VI regulates the spatial organisation of mammalian transcription initiation.
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Abstract

During transcription, RNA Polymerase II (RNAPII) is spatially organised within the nucleus into clusters that correlate with transcription activity. While this is a hallmark of genome regulation in mammalian cells, the mechanisms concerning the assembly, organisation and stability remain unknown. Here, we have used combination of single molecule imaging and genomic approaches to explore the role of nuclear myosin VI (MVI) in the nanoscale organisation of RNAPII. We reveal that MVI in the nucleus acts as the molecular anchor that holds RNAPII in high density clusters. Perturbation of MVI leads to the disruption of RNAPII localisation, chromatin organisation and subsequently a decrease in gene expression. Overall, we uncover the fundamental role of MVI in the spatial regulation of gene expression.

Keywords:RNA Polymerase II, Myosin VI, gene expression, spatial organisation
Subjects:C Biological Sciences > C130 Cell Biology
Divisions:College of Science > School of Life Sciences
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ID Code:49719
Deposited On:08 Jun 2022 13:49

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