High-throughput method for the extraction, isolation and identification of antifungal compounds from plants

Mortimer, Megan (2020) High-throughput method for the extraction, isolation and identification of antifungal compounds from plants. Masters thesis, University of Lincoln.

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High-throughput method for the extraction, isolation and identification of antifungal compounds from plants
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Mortimer, Megan-Life Sciences- September 2020.pdf - Whole Document

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Item Type:Thesis (Masters)
Item Status:Live Archive

Abstract

This research aims to develop a high-throughput, standardised methodology for the extraction, isolation and identification of antifungal compounds from plants, using a bottom-up approach. The method development began with screening strains of non-pathogenic fungi Saccharomyces cerevisiae spp., and Komagataella phaffii, against solvent extractions from 22 different plants from various families in the classic 96-well microtitre Minimum Inhibitory Concentration (MIC) test. Plant extracts which displayed activity in the MIC test were then analysed with HPLC and HPLC-MS. One plant tested (Corema album) displayed significant activity against Candida albicans and Candida auris. This method identified three potential compounds, pinocembrin, 2,4-dihydroxychalcone and 7-hydroxyflavone. Pinocembrin gave an IC50 of 31.25 µg/ml against C. auris and should be considered for further pharmacological assessment.

Divisions:College of Science > School of Life Sciences
ID Code:47817
Deposited On:18 Jan 2022 10:33

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