NDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription.

Fili, Natalia, Hari-Gupta, Yukti, Dos Santos, Ália , Cook, Alexander, Poland, Simon, Ameer-Beg, Simon M., Parsons, Maddy and Toseland, Christopher P. (2017) NDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription. Nature Communications, 8 (1). p. 1871. ISSN 2041-1723

Full content URL: https://doi.org/10.1038/s41467-017-02050-w

Full text not available from this repository.

Item Type:Article
Item Status:Live Archive


Myosin VI (MVI) has been found to be overexpressed in ovarian, breast and prostate cancers. Moreover, it has been shown to play a role in regulating cell proliferation and migration, and to interact with RNA Polymerase II (RNAPII). Here, we find that backfolding of MVI regulates its ability to bind DNA and that a putative transcription co-activator NDP52 relieves the auto-inhibition of MVI to enable DNA binding. Additionally, we show that the MVI-NDP52 complex binds RNAPII, which is critical for transcription, and that depletion of NDP52 or MVI reduces steady-state mRNA levels. Lastly, we demonstrate that MVI directly interacts with nuclear receptors to drive expression of target genes, thereby suggesting a link to cell proliferation and migration. Overall, we suggest MVI may function as an auxiliary motor to drive transcription.

Keywords:myosin VI, gene expression, NDP52, RNA polymerase II
Subjects:C Biological Sciences > C130 Cell Biology
Divisions:College of Science > School of Life and Environmental Sciences > Department of Life Sciences
Related URLs:
ID Code:46353
Deposited On:06 Jan 2023 15:54

Repository Staff Only: item control page