Comparison of Compact Dry and Conventional ISO Methods for Microbiological Survey of Lettuce Varieties at Retail Stage

Aiyedun, Susanna, Swainson, Mark, Dixon, Ron and Onarinde, Bukola (2021) Comparison of Compact Dry and Conventional ISO Methods for Microbiological Survey of Lettuce Varieties at Retail Stage. In: IAFP European Symposium on Food Safety, 27 - 28 April 2021.

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Item Type:Conference or Workshop contribution (Poster)
Item Status:Live Archive

Abstract

Introduction: Prevalence of foodborne outbreaks linked with fresh produce consumption calls for exploring rapid methods to investigate diverse microbial communities associated with fresh produce.
Purpose: This study evaluated the performance of compact dry and conventional methods in determining the microbial ecology of lettuce varieties at retail stage.

Methods: Microbiological analysis was carried out using compact dry and conventional methods for 7 lettuce varieties (including cos, curly leaf, iceberg, little gem, romaine hearts, round and sweet gem). Samples were analysed for aerobic bacteria, yeasts and moulds, coliforms and Enterobacteriaceae while pathogen levels were determined for Salmonella spp., Staphylococcus aureus and Listeria monocytogenes.

Results: Using conventional methods, mean microbial counts ranged from 6.1 x 103 CFU/g to 1.4 x 106 CFU/g (aerobic bacteria); 3.2 x 103 CFU/g to 1.7 x 105 CFU/g (yeasts and moulds); 1.2 x 102 CFU/g to 1.3 x 105 CFU/g (Enterobacteriaceae); 1.0 x 102 CFU/g to 1.1 x 105 CFU/g (coliforms); 0.0 CFU/g to 3.0 x 103 CFU/g (Salmonella spp.); 0.0 CFU/g to 1.3 x 104 CFU/g (Staphylococcus aureus) and 6.0 x 102CFU/g CFU/g to 1.0 x 105 CFU/g (Listeria monocytogenes). However, mean microbial counts using compact dry methods ranged from 6.0 x 103 CFU/g to 1.5 x 106 CFU/g (aerobic bacteria); 2.8 x 103 CFU/g to 1.7 x 105 CFU/g (yeasts and moulds); 1.2 x 102 CFU/g to 1.1 x 105 CFU/g (Enterobacteriaceae); 1.2 x 102 CFU/g to 1.0 x 105CFU/g (coliforms); 0.0 CFU/g to 2.0 x 103 CFU/g (Salmonella spp.); 0.0 CFU/g to 1.2 x 104 CFU/g (Staphylococcus aureus) and 3.0 x 102 CFU/g to 1.0 x 105 CFU/g (Listeria monocytogenes). Furthermore, mean microbial counts of both methods revealed a positive correlation (r = 0.9593).

Significance: Compact dry method is a reliable alternative for timely investigation of microbial communities associated with fresh produce like lettuce.

Keywords:Lettuce, Compact dry, Conventional ISO methods, Indicator bacteria, Pathogens
Subjects:D Veterinary Sciences, Agriculture and related subjects > D610 Food Science
C Biological Sciences > C500 Microbiology
Divisions:College of Science > National Centre for Food Manufacturing
ID Code:44293
Deposited On:31 Mar 2021 10:51

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