Oriented Immobilization on Gold Nanoparticles of a Recombinant Therapeutic Zymogen

Dosadina, Elina, Agyeiwaa, Celetia, Ferreira, William , Cutting, Simon, Jibawi, Abdullah, Ferrari, Enrico and Soloviev, Mikhail (2020) Oriented Immobilization on Gold Nanoparticles of a Recombinant Therapeutic Zymogen. In: Nanoparticles in Biology and Medicine. Springer, pp. 213-225. ISBN 978-1-0716-0319-2

Full content URL: https://doi.org/10.1007/978-1-0716-0319-2_16

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Item Type:Book Section
Item Status:Live Archive


Direct immobilization of functional proteins on gold nanoparticles (AuNPs) affects their structure and function. Changes may vary widely and range from strong inhibition to the enhancement of protein function. More often though the outcome of direct protein immobilization results in protein misfolding and the loss of protein activity. Additional complications arise when the protein being immobilized is a zymogen which requires and relies on additional protein--protein interactions to exert its function. Here we describe molecular design of a glutathione-S-transferase-Staphylokinase fusion protein (GST-SAK) and its conjugation to AuNPs. The multivalent AuNP-(GST-SAK)n complexes generated show plasminogen activation activity in vitro. The methods described are transferable and could be adapted for conjugation and functional analysis of other plasminogen activators, thrombolytic preparations or other functional enzymes.

Subjects:B Subjects allied to Medicine > B990 Subjects Allied to Medicine not elsewhere classified
Divisions:College of Science > School of Life Sciences
ID Code:40210
Deposited On:15 Apr 2020 13:21

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