Evaluation of five preservation methods for recovery of DNA from bone

Iyavoo, Sasitaran, Hadi, Sibte and Goodwin, William (2019) Evaluation of five preservation methods for recovery of DNA from bone. Forensic Science International: Genetics Supplement Series, 7 (1). pp. 200-202. ISSN 1875-1768

Full content URL: https://doi.org/10.1016/j.fsigss.2019.09.077

Full text not available from this repository.

Item Type:Article
Item Status:Live Archive


The effectiveness of five methods for the preservation of bone were assessed: cell lysis solution (with 1% sodium azide), dehydration/freeze-drying, ethanol (96%), freezing, and room temperature storage. Preserved bone samples were extracted using five optimised extraction methods. These preservation methods were tested for their efficiency for storage of fresh and degraded bone samples for 6 weeks, 6 months and 1 year. Freezing was found to be the best preservation method for longer-term storage of bone samples; this was followed by ethanol (96%), dehydration/freeze drying, and room temperature storage. Full profiles were obtained from bone samples using all these preservation methods.

Keywords:DNA, Bone, Preservation, PCR inhibition, Degradation
Subjects:F Physical Sciences > F410 Forensic Science
Divisions:College of Science > School of Chemistry
ID Code:39386
Deposited On:08 Jan 2020 09:01

Repository Staff Only: item control page