Evaluation of decalcification for recovery of DNA from bone

Iyavoo, Sasitaran, Hadi, Sibte and Goodwin, William (2017) Evaluation of decalcification for recovery of DNA from bone. Forensic Science International: Genetics Supplement Series, 6 . e270-e272. ISSN 1875-1768

Full content URL: http://doi.org/10.1016/j.fsigss.2017.09.087

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Item Type:Article
Item Status:Live Archive


The effect of decalcification on pulverised bone samples was studied to evaluate its value when processing reasonably good quality samples. The decalcified and non-decalcified bone samples were extracted using phenolchloroform extraction method, quantitated with GoTaq® qPCR Master Mix (Promega®, UK) using Applied Biosystems® 7500 Real-Time PCR System (Thermo Fisher Scientific®, UK) and amplified using an in-house amplification multiplex. The results showed that the decalcification of bone samples is not a necessary when processing good quality samples and higher DNA yields were obtained without the decalcification process. The electropherograms produced from the extracted DNA samples without decalcification were good quality and displayed no PCR inhibition.

Keywords:DNA, Bone, Decalcification, Inhibition
Subjects:F Physical Sciences > F410 Forensic Science
Divisions:College of Science > School of Chemistry
ID Code:39172
Deposited On:23 Dec 2019 11:37

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