Iyavoo, Sasitaran, Cummings, Steve, Wolejko, Agata , Furmanczyk, Dagmara, Graham, Richard, Myers, Rosie and Haizel, Thomas (2015) Reduced volume PCR amplification using AmpFℓSTR ® Identifiler ® kit. Forensic Science International: Genetics Supplement Series, 5 . e398-e399. ISSN 1875-1768
Full content URL: http://doi.org/10.1016/j.fsigss.2015.09.157
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| Item Type: | Article |
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| Item Status: | Live Archive |
Abstract
This study was aimed to demonstrate that the AmpFlSTR® Identifiler® kit will reliably amplify DNA in a reaction volume of 10ml. For comparison, the DNA samples were also amplified at the currect in house PCR reaction volume (12.5ml). Even though the results showed significant difference in the peak height intensities, the quality of the profiles produced in the reaction volume of 10ml was similar to those produced in the reaction volume of 12.5ml. The application of this reduced volume PCR amplification would represent an additional 20% cost saving on the reagents without compromising the quality of the profiles obtained.
| Keywords: | DNA, PCR, STR, Reduced volume, Identifiler |
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| Subjects: | F Physical Sciences > F410 Forensic Science |
| Divisions: | College of Science > School of Chemistry |
| ID Code: | 39166 |
| Deposited On: | 23 Dec 2019 11:33 |
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