Reduced PCR cycling time amplification using AmpFℓSTR ® Identifiler ® kit

Iyavoo, Sasitaran, Wolejko, Agata, Furmanczyk, Dagmara , Graham, Richard, Myers, Rosie and Haizel, Thomas (2015) Reduced PCR cycling time amplification using AmpFℓSTR ® Identifiler ® kit. Forensic Science International: Genetics Supplement Series, 5 . e286-e288. ISSN 1875-1768

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To reduce the DNA profiling time, DNA samples were amplified using AmpFlSTR® Identifiler® kit at reduced PCR cycling time amplification method without addition of any enhancement buffers or enzymes. The results showed no significant difference in the quality of the profiles produced compared to those produced in the manufacturer recommended condition. This study contributed in saving almost 25% of the time needed for PCR amplification without compromising the quality of the profiles.

Keywords:DNA, PCR, STR, Reduced time, Identifiler
Subjects:F Physical Sciences > F410 Forensic Science
Divisions:College of Science > School of Chemistry
ID Code:39165
Deposited On:23 Dec 2019 11:29

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