Iyavoo, Sasitaran, Hadi, Sibte and Goodwin, William (2013) Evaluation of five DNA extraction systems for recovery of DNA from bone. Forensic Science International: Genetics Supplement Series, 4 (1). e174-e175. ISSN 1875-1768
Full content URL: http://doi.org/10.1016/j.fsigss.2013.10.090
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| Item Type: | Article |
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| Item Status: | Live Archive |
Abstract
Five DNA extraction systems were assessed for their DNA extraction efficiency on samples of fresh pig bone. Four commercially available silica-based extraction kits (ChargeSwitch® gDNA Plant Kit (Life Technologies), DNA IQ® System Kit (Promega), DNeasy® Blood & Tissue Kit (Qiagen) and PrepFiler® BTA Forensic DNA Extraction Kit (Life Technologies)) and a conventional phenol-chloroform method were tested in this study. Extracted DNA samples were quantitated with GoTaq® qPCR Master Mix (Promega) using an Applied Biosystems® 7500 Real-Time PCR System and the extracts were amplified using an inhouse multiplex system. The phenol-chloroform extraction produced higher yields of DNA than the silica-based extraction methods. Among the silica-based extractions ChargeSwitch® gDNA Plant Kit recovered the highest amounts of DNA. However, all methods produced DNA that could be amplified and none of the extracts contained any detectable inhibition.
| Keywords: | DNA extraction, Bone, Silica-based extraction, Forensic |
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| Subjects: | F Physical Sciences > F410 Forensic Science |
| Divisions: | College of Science > School of Chemistry |
| ID Code: | 39163 |
| Deposited On: | 23 Dec 2019 11:27 |
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