Evaluation of five DNA extraction systems for recovery of DNA from bone

Iyavoo, Sasitaran, Hadi, Sibte and Goodwin, William (2013) Evaluation of five DNA extraction systems for recovery of DNA from bone. Forensic Science International: Genetics Supplement Series, 4 (1). e174-e175. ISSN 1875-1768

Full content URL: http://doi.org/10.1016/j.fsigss.2013.10.090

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Item Type:Article
Item Status:Live Archive


Five DNA extraction systems were assessed for their DNA extraction efficiency on samples of fresh pig bone. Four commercially available silica-based extraction kits (ChargeSwitch® gDNA Plant Kit (Life Technologies), DNA IQ® System Kit (Promega), DNeasy® Blood & Tissue Kit (Qiagen) and PrepFiler® BTA Forensic DNA Extraction Kit (Life Technologies)) and a conventional phenol-chloroform method were tested in this study. Extracted DNA samples were quantitated with GoTaq® qPCR Master Mix (Promega) using an Applied Biosystems® 7500 Real-Time PCR System and the extracts were amplified using an inhouse multiplex system. The phenol-chloroform extraction produced higher yields of DNA than the silica-based extraction methods. Among the silica-based extractions ChargeSwitch® gDNA Plant Kit recovered the highest amounts of DNA. However, all methods produced DNA that could be amplified and none of the extracts contained any detectable inhibition.

Keywords:DNA extraction, Bone, Silica-based extraction, Forensic
Subjects:F Physical Sciences > F410 Forensic Science
Divisions:College of Science > School of Chemistry
ID Code:39163
Deposited On:23 Dec 2019 11:27

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