Examining Cell-Cell Interactions in the Kidney Using AFM Single-Cell Force Spectroscopy

Siamantouras, Eleftherios, Hills, Claire, Liu, Kuo-Kang and Squires, Paul (2020) Examining Cell-Cell Interactions in the Kidney Using AFM Single-Cell Force Spectroscopy. In: Diabetic Nephropathy. Methods in Molecular Biology, 2067 . Humana Press, Humana, New York, NY, pp. 189-201. ISBN 978-1-4939-9840-1, 978-1-4939-9841-8

Full content URL: https://doi.org/10.1007/978-1-4939-9841-8_14

Examining cell-cell interactions in the kidney using AFM single-cell force spectroscopy
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The ability of individual cells to synchronize activity is a basic feature of efficient and appropriate tissue function. Central to this is the physicochemical binding between cells through multiprotein complexes that functionally mediate adhesion. Importantly, the direct connection of physical properties and intercellular signaling is of great importance to certain pathologies including diabetes. Atomic force microscopy (AFM) single-cell force spectroscopy (SCFS) is a high-resolution technique that provides a statistically reliable measurement of the minute forces involved in cell tethering and membrane dynamics. Detection of altered nanoscale forces underlying the loss of adhesion in early tubular injury is pivotal for the development of novel therapeutic strategies for diabetic nephropathy. Here we describe the step-by-step use of an integrated AFM-SCFS system designed to measure functional force-displacement in separating renal tubular epithelial cells. Parameters such as unbinding forces, detachment energy, and distance to complete separation can be obtained from force-displacement (F-d) curves and are critical in assessing how physical changes of cellular adhesion contribute to cell contact, coupling, and communication in the diabetic kidney.

Keywords:AFM, Cadherin, cell adhesion, cell-cell coupling, membrane dynamics, nanomedicine
Subjects:A Medicine and Dentistry > A100 Pre-clinical Medicine
B Subjects allied to Medicine > B120 Physiology
C Biological Sciences > C130 Cell Biology
C Biological Sciences > C770 Biophysical Science
Divisions:College of Science > School of Life Sciences
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ID Code:39041
Deposited On:07 Feb 2020 13:30

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