Isolation and characterisation of thermophilic micro-organisms able to grow on cellulose acetate

Degli-Innocenti, Francesco, Goglino, Giorgia, Tosin, Maurizio , Bellia, Gaetano, Monciardini, P and Cavalletti, L (2002) Isolation and characterisation of thermophilic micro-organisms able to grow on cellulose acetate. In: Microbiology of Composting. Springer, pp. 273-286. ISBN UNSPECIFIED

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Cellulose acetate (CA) can be successfully used for producing compostable plastics. It is biodegradable under composting conditions, but neither the mechanism of degradation nor the involved microorganisms are known. We isolated five thermophilic strains from compost which were identified by molecular characterisation as belonging to the family Thermomonosporaceae, probably to the genus Actinomadura. The strains are able to grow at 50 °C on minimal plates supplemented with CA (DS=2) or microcrystalline cellulose in powder. The degradation of CA is visible by the clarification of the area surrounding the mycelium. The strains grow at 37 °C on cellulose but not on CA. At 20 and 58 °C no growth is detected on both substrates. The strains are viable after a 2-week incubation period at 65 °C. Neutral and basic pHs are the best for CA degradation, while poor results are obtained under acidic conditions. The best degradation of CA is obtained using nitrate as nitrogen source. Solid-state respiration tests, performed using sterile vermiculite as a matrix, confirmed that the strains are able to mineralise the CA. The strains failed to grow in CA under liquid conditions.

Keywords:Minimal Medium, Mature Compost, Microcrystalline Cellulose, Cellulose Acetate
Subjects:C Biological Sciences > C110 Applied Biology
C Biological Sciences > C500 Microbiology
F Physical Sciences > F162 Polymer Chemistry
Divisions:College of Science > School of Pharmacy
ID Code:36482
Deposited On:18 Jul 2019 13:05

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