Changes in activities of enzymes of carbon metabolism in leaves during exposure of plants to low temperature

Holaday, A.S., Martindale, Wayne, Aired, R. , Brooks, A.L. and Leegood, R.C. (1992) Changes in activities of enzymes of carbon metabolism in leaves during exposure of plants to low temperature. Plant Physiology, 98 (3). pp. 1105-1114. ISSN 00320889

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The aim of this study was to determine the response of photosynthetic carbon metabolism in spinach and bean to low temperature, (a) Exposure of warm-grown spinach and bean plants to 10°C for 10 days resulted in increases in the total activities of a number of enzymes, including ribulose 1,5-bisphosphate carboxylase (Rubisco), stromal fructose 1,6 bisphosphatase (Fru 1,6-P2ase), sedoheptulose 1,7-bisphosphatase (Sed 1,7-P2ase), and the cytosolic Fru 1,6-P2ase. In spinach, but not bean, there was an increase in the total activity of sucrose-phosphate synthase. (b) The CO2-saturated rates of photosynthesis for the colda-cclimated spinach plants were 68 greater at 10°C than those for warm-acclimated plants, whereas in bean, rates of photosynthesis at 10°C were very low after exposure to low temperature, (c) When spinach leaf discs were transferred from 27 to 10°C, the stromal Fru 1,6-P2ase and NAOP-malate dehydrogenase were almost fully activated within 8 minutes, and Rubisco reached 90 of full activation within 15 minutes of transfer. An initial restriction of Calvin cycle fluxes was evident as an increase in the amounts of ribulose 1,5-bisphosphate, glycerate-3-phosphate, Fru 1,6-P2, and Sed 1,7-P2. In bean, activation of stromal Fru 1,6-P2ase was weak, whereas the activation state of Rubisco decreased during the first few minutes after transfer to low temperature. However, NADP-malate dehydrogenase became almost fully activated, showing that no loss of the capacity for reductive activation occurred, (d) Temperature compensation in spinach evidently involves increases in the capacities of a range of enzymes, achieved in the short term by an increase in activation state, whereas long-term acclimation is achieved by an increase in the maximum activities of enzymes. The inability of bean to activate fully certain Calvin cycle enzymes and sucrose-phosphate synthase, or to increase nonphotochemical quenching of chlorophyll fluorescence at 10°C, may be factors contributing to its poor performance at low temperature.

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Subjects:C Biological Sciences > C210 Applied Botany
C Biological Sciences > C230 Plant Biotechnology
Divisions:College of Science > National Centre for Food Manufacturing
ID Code:33779
Deposited On:17 Oct 2018 08:18

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