Abstract

A new, selective and sensitive spectrofluorimetric method was developed and validated for the
determination of perindopril erbumine via derivatization with o-phthalaldehyde in presence of 3-
mercaptopropionic acid in alkaline medium. The resulted derivative has a strong blue fluorescence that was
measured at 452 nm after excitation at 340 nm in aqueous solution. The reaction conditions were carefully studied and optimized. Under the optimum conditions, the fluorescence intensity was linear over a concentration range of 1.2 – 8.4 ug/mL (R2 = 0.9997) with a detection limit of 0.097ug/mL. The proposed method was fully validated and successfully applied to the analysis of perindopril erbumine in pure form and tablets. Statistical comparison of the results obtained by the proposed and reference method revealed no significant difference in the performance of the two methods regarding the accuracy and precision respectively. A proposal for the reaction pathway with o-phthalaldehyde was postulated.