A three-Locus, PCR based method for forensic Identification of plant material

Srivastava, Tushar, Wu, Michael, Kakhnovich, Julia , Waithaka, Bridgit and Lents, Nathan H. (2018) A three-Locus, PCR based method for forensic Identification of plant material. Journal of Forensic Sciences, 63 (4). pp. 1252-1260. ISSN 0022-1198

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Plant residue is currently an underutilized resource in forensic investigations despite the fact that many crime scenes, as well as suspects and victims, harbor plant-derived residue that could be recovered and analyzed. Notwithstanding the considerable skill of forensic botanists, current methods of species determination could benefit from tools for DNA-based species identification. However, DNA barcoding in plants has been hampered by sequence complications in the plant genome. Following a database search for usable barcodes, broad-spectrum primers were designed and utilized to amplify and sequence the rbcL, trnL-F, and rrn18 genetic loci from a variety of household plants. Once obtained, these DNA sequences were used to design species-targeted primers that could successfully discriminate the source of plant residue from among the 21 species tested. [Abstract copyright: © 2017 American Academy of Forensic Sciences.]

Additional Information:** From PubMed via Jisc Publications Router. ** History: received 17-08-2017; revised 07-10-2017; revised 11-11-2017; accepted 15-11-2017.
Keywords:orensic science, botany, forensic botany, forensic biology, DNA barcodes, DNA barcoding, pollen, plants, polymerase chainreaction
Subjects:F Physical Sciences > F180 Analytical Chemistry
F Physical Sciences > F410 Forensic Science
Divisions:College of Science > School of Life Sciences
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ID Code:29988
Deposited On:21 Mar 2018 17:23

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