Reduced levels of dopamine and altered metabolism in brains of HPRT knock-out rats: a new rodent model of Lesch-Nyhan Disease

Meek, Stephen, Thomson, Alison J., Sutherland, Linda , Sharp, Matthew G. F., Thomson, Julie, Bishop, Valerie, Meddle, Simone L., Gloaguen, Yoann, Weidt, Stefan, Singh-Dolt, Karamjit, Buehr, Mia, Brown, Helen K., Gill, Andrew C. and Burdon, Tom (2016) Reduced levels of dopamine and altered metabolism in brains of HPRT knock-out rats: a new rodent model of Lesch-Nyhan Disease. Scientific Reports, 6 (1). p. 25592. ISSN 2045-2322

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Lesch-Nyhan disease (LND) is a severe neurological disorder caused by loss-of-function mutations in the gene encoding hypoxanthine phosphoribosyltransferase (HPRT), an enzyme required for efficient recycling of purine nucleotides. Although this biochemical defect reconfigures purine metabolism and leads to elevated levels of the breakdown product urea, it remains unclear exactly how loss of HPRT activity disrupts brain function. As the rat is the preferred rodent experimental model for studying neurobiology and diseases of the brain, we used genetically-modified embryonic stem cells to generate an HPRT knock-out rat. Male HPRT-deficient rats were viable, fertile and displayed normal caged behaviour. However, metabolomic analysis revealed changes in brain biochemistry consistent with disruption of purine recycling and nucleotide metabolism. Broader changes in brain biochemistry were also indicated by increased levels of the core metabolite citrate and reduced levels of lipids and fatty acids. Targeted MS/MS analysis identified reduced levels of dopamine in the brains of HPRT-deficient animals, consistent with deficits noted previously in human LND patients and HPRT knock-out mice. The HPRT-deficient rat therefore provides a new experimental platform for future investigation of how HPRT activity and disruption of purine metabolism affects neural function and behaviour.

Keywords:Lesch-Nyhan disease, Metabolomics, Dopamine, Serotonin, HPRT
Subjects:C Biological Sciences > C730 Metabolic Biochemistry
C Biological Sciences > C120 Behavioural Biology
C Biological Sciences > C140 Developmental/Reproductive Biology
Divisions:College of Science
ID Code:29463
Deposited On:14 Nov 2017 15:18

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