Monitoring dental-unit-water-line output water by current in-office test kits

Lal, S., Singhrao, S. K., Bricknell, M. , Pearce, M., Morton, L. H. G., Ahmed, W. and Crean, St.J. (2014) Monitoring dental-unit-water-line output water by current in-office test kits. Current Microbiology, 69 (2). pp. 135-142. ISSN 0343-8651

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The importance of monitoring contamination levels in the output water of dental-unit-water-lines (DUWLs) is essential as they are prone to developing biofilms that may contaminate water that is used to treat patients, with opportunistic pathogens such as species of Legionella, Pseudomonas and others. Dentists and practice staff are also at risk of being infected by means of cross-infection due to aerosols generated from DUWL water. The unit of measurement for the microbial contamination of water by aerobic mesophilic heterotrophic bacteria is the colony-forming unit per millilitre (cfu/ml) of water. The UK has its own guidelines set by the Department of Health for water discharged from DUWL to be between 100 and 200 cfu/ml of water. The benchmark or accepted standard laboratory test is by microbiological culture on R2A agar plates. However, this is costly and not convenient for routine testing in dental practices. A number of commercial indicator tests are used in dental surgeries, but they were not developed for the dental market and serve only to indicate gross levels of contamination when used outside of the manufacturer's recommended incubation period. The aim of this article is to briefly review the universal problem of DUWL contamination with microbial biofilms and to update dental professionals on the availability of currently available commercial in-office monitoring systems for aerobic mesophilic heterotrophic bacteria and to discuss their limitations for testing water samples in assuring compliance with recommended guidelines. © 2014 Springer Science+Business Media.

Keywords:agar, drinking water, endotoxin, water, agar medium, bacterial count, bacterial load, bacterium contamination, biofilm, colony forming unit, dental practice, dental procedure, dentistry, disease transmission, health hazard, heterotrophy, human, incubation time, infection control, Legionella, mesophilic bacterium, microbiological examination, monitoring, Mycobacterium, nonhuman, occupational health, plate count, practice guideline, priority journal, Pseudomonas, quorum sensing, rapid test, review, syringe, ultrasonic scaler, water analysis, water contamination, water flow, water sampling, aerobic bacterium, dental facility, hospital information system, isolation and purification, microbiology, procedures, United Kingdom, Legionella, Pseudomonas, Bacteria, Aerobic, Bacteriological Techniques, Dental Offices, Great Britain, Point-of-Care Systems, Water Microbiology, NotOAChecked
Divisions:College of Science > School of Mathematics and Physics
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ID Code:27138
Deposited On:05 Jul 2017 13:16

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