A chemically defined carrier for the delivery of human mesenchymal stem/stromal cells to skin wounds

Walker, Nathan G., Mistry, Anita R., Smith, Louise E. , Eves, Paula, Tsaknakis, Grigorios, Forster, Simon, Watt, Suzanne M. and MacNeil, Sheila (2012) A chemically defined carrier for the delivery of human mesenchymal stem/stromal cells to skin wounds. Tissue Engineering - Part C: Methods, 18 (2). pp. 143-155. ISSN 1937-3384

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Skin has a remarkable capacity for regeneration, but age-and diabetes-related vascular problems lead to chronic non-healing wounds for many thousands of U.K. patients. There is a need for new therapeutic approaches to treat these resistant wounds. Donor mesenchymal stem/stromal cells (MSCs) have been shown to assist cutaneous wound healing by accelerating re-epithelialization. The aim of this work was to devise a low risk and convenient delivery method for transferring these cells to wound beds. Plasma polymerization was used to functionalize the surface of medical-grade silicone with acrylic acid. Cells attached well to these carriers, and culture for up to 3 days on the carriers did not significantly affect their phenotype or ability to support vascular tubule formation. These carriers were then used to transfer MSCs onto human dermis. Cell transfer was confirmed using an MTT assay to assess viable cell numbers and enhanced green fluorescent protein-labeled MSCs to demonstrate that the cells post-transfer attached to the dermis. We conclude that this synthetic carrier membrane is a promising approach for delivery of therapeutic MSCs and opens the way for future studies to evaluate its impact on repairing difficult skin wounds. © 2012 Mary Ann Liebert, Inc.

Keywords:Craboxylic acids, Cells, Flowcharting, Functional polymers, Plasma polymerization, Silicones, Acrylic acids, Cell transfer, Cutaneous wounds, Delivery methods, Human dermis, MTT assays, Re-epithelialization, Viable cells
Subjects:C Biological Sciences > C131 Applied Cell Biology
H Engineering > H673 Bioengineering
Divisions:Professional services
ID Code:26983
Deposited On:21 Apr 2017 08:47

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