Obesity and sex influence insulin resistance and total and multimer adiponectin levels in adult neutered domestic shorthair client-owned cats

Bjornvad, C. R., Rand, J. S., Tan, H. Y. , Jensen, K. S., Rose, F. J., Armstrong, P. J. and Whitehead, J. P. (2014) Obesity and sex influence insulin resistance and total and multimer adiponectin levels in adult neutered domestic shorthair client-owned cats. Domestic Animal Endocrinology, 47 (1). pp. 55-64. ISSN 0739-7240


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In this study, we estimated insulin sensitivity and determined plasma concentrations of total-, low-molecular-weight (LMW), and high-molecular-weight (HMW) adiponectin and leptin in 72 domestic shorthair, neutered, client-owned cats. Glucose tolerance was assessed with an intravenous glucose tolerance test and body fat percentage (BF) was measured with dual-energy x-ray absorptiometry. Total adiponectin was measured with 2 different ELISAs. Low-molecular-weight and HMW adiponectin plasma concentrations were determined by Western blot analysis after sucrose-gradient velocity centrifugation, and the adiponectin multimer ratio SA = HMW/(HMW + LMW) was calculated. Differences in glucose tolerance, leptin, total adiponectin, and multimer ratio among lean (BF% <35; n = 26), overweight (35 <BF% <45; n = 28), and obese (BF% >45; n = 18) cats as well as between male (n = 34) and female (n = 38) neutered cats were evaluated by linear regression and 2-way ANOVA. Sex and age were included as covariates for analysis of BF%, whereas BF%, fat mass, and lean body mass were covariates for analysis of sex differences. Increased BF% was negatively correlated with multimer ratio (SA, r = -45; P < 0.002), whereas no differences were found in total adiponectin concentrations among BF% groups (P > 0.01). Male cats had indices of decreased insulin tolerance and significantly lower total adiponectin concentrations than did female cats (mean ± SEM, 3.7 ± 0.4 vs 5.4 ± 0.5 μg/mL; P < 0.02). Altered SAs could contribute to an obesity-associated decreasing glucose tolerance in cats, and low total adiponectin concentrations may relate to increased risk of diabetes mellitus in neutered male cats. © 2014 Elsevier Inc.

Keywords:Felidae, adipocytokine, adiponectin, animal, animal disease, article, blood, cat, cat disease, chemistry, dual-energy x-ray absorptiometry, Feline, female, gender, genetics, insulin resistance, male, metabolism, obesity, physiology, sex difference, Adipokine, Gender, Adiponectin, Animals, Cat Diseases, Cats, Sex Factors
Subjects:D Veterinary Sciences, Agriculture and related subjects > D322 Animal Physiology
D Veterinary Sciences, Agriculture and related subjects > D100 Pre-clinical Veterinary Medicine
Divisions:College of Science > School of Life Sciences
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ID Code:26211
Deposited On:14 Mar 2018 16:47

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