Induction of heme-oxygenase-1 (HO-1) does not enhance adiponectin production in human adipocytes: evidence against a direct HO-1 - Adiponectin axis

Yang, Mengliu, Kimura, Masaki, Ng, Choaping , He, Jingjing, Keshvari, Sahar, Rose, Felicity J., Barclay, Johanna L. and Whitehead, Jonathan P. (2015) Induction of heme-oxygenase-1 (HO-1) does not enhance adiponectin production in human adipocytes: evidence against a direct HO-1 - Adiponectin axis. Molecular and Cellular Endocrinology, 413 . pp. 209-216. ISSN 0303-7207


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Adiponectin is a salutary adipokine and hypoadiponectinemia is implicated in the aetiology of obesity-related inflammation and cardiometabolic disease making therapeutic strategies to increase adiponectin attractive. Emerging evidence, predominantly from preclinical studies, suggests induction of heme-oxygenase-1 (HO-1) increases adiponectin production and reduces inflammatory tone. Here, we aimed to test whether induction of HO-1 enhanced adiponectin production from mature adipocytes. Treatment of human adipocytes with cobalt protoporphyrin (CoPP) or hemin for 24-48 h increased HO-1 expression and activity without affecting adiponectin expression and secretion. Treatment of adipocytes with TNFα reduced adiponectin secretion and increased expression and secretion of additional pro-inflammatory cytokines, IL-6 and MCP-1, as well as expression of sXBP-1, a marker of ER stress. HO-1 induction failed to reverse these effects. These results demonstrate that induction of HO-1 does not directly enhance adiponectin production or ameliorate the pro-inflammatory effects of TNFα and argue against a direct HO-1 - adiponectin axis. © 2015 Elsevier Ireland Ltd.

Keywords:adiponectin, heme oxygenase 1, interleukin 6, monocyte chemotactic protein 1, tumor necrosis factor alpha, X box binding protein 1, ADIPOQ protein, human, CCL2 protein, human, HMOX1 protein, human, IL6 protein, human, protoporphyrin, protoporphyrin cobalt, tumor necrosis factor alpha, adipocyte, Article, cell differentiation, cell isolation, cell stress, controlled study, cytokine production, enzyme activity, enzyme induction, human, human cell, priority journal, proadipocyte, protein determination, protein expression, protein secretion, adipocyte, biosynthesis, cell culture, cytology, drug effects, metabolism, physiology, Adipocytes, Cells, Cultured, Chemokine CCL2, Heme Oxygenase-1, Humans, Interleukin-6, Protoporphyrins, Tumor Necrosis Factor-alpha
Subjects:B Subjects allied to Medicine > B120 Physiology
C Biological Sciences > C130 Cell Biology
Divisions:College of Science > School of Life Sciences
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ID Code:26202
Deposited On:06 Mar 2018 08:33

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