Expression of OP4 (ORL1, NOP1) receptors in vascular endothelium

Granata, Filippo, Potenza, Rossella Luisa, Fiori, Anna , Strom, Roberto, Caronti, Brunella, Molinari, Paola, Donsante, Sonia, Citro, Gennaro, Iacovelli, Luisa, De Blasi, Antonio, Ngomba, Richard T., Palladini, Guido and Passarelli, Francesca (2003) Expression of OP4 (ORL1, NOP1) receptors in vascular endothelium. European Journal of Pharmacology, 482 (1-3). pp. 17-23. ISSN 0014-2999

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Endothelial cells from rat brain microvessels, human aortic artery and human umbilical vein were examined, together Endothelial cells from rat brain microvessels, human aortic artery and human umbilical vein were examined, together with ex vivo rat brain capillaries and rat aortic ring sections, for the expression of opioid receptor-like OP-4 mRNA and protein. High levels of mRNA expression and an immunopositive reaction for the receptor protein were detected in the endothelial cells from primary and from established in vitro cultures, as well as in the intima of ex vivo rat aortic rings, where the signal was limited to the endothelial layer. Interaction of the OP4 receptor with its physiological ligand nociceptin caused, in cultured endothelial cells, the activation of a mitogen-activated protein (MAP) kinase cascade. Taken together, these results show that the OP4 receptor is synthesised and functionally expressed in endothelial cells, presumably as a starting point for some vasoactive mechanism(s).

Keywords:messenger RNA, mitogen activated protein kinase, nociceptin, opiate receptor, opioid like receptor 1, receptor protein, unclassified drug, animal cell, animal tissue, aorta, article, brain blood vessel, brain capillary, cell culture, controlled study, endothelium cell, enzyme activation, ex vivo study, human, human cell, immune response, in vitro study, male, microvasculature, nonhuman, priority journal, protein expression, protein protein interaction, protein synthesis, rat, signal transduction, umbilical vein, vascular endothelium, vascular ring, Animals, Aorta, Thoracic, Brain, Carrier Proteins, Cercopithecus aethiops, COS Cells, Endothelium, Vascular, Fungal Proteins, Gene Expression Regulation, Humans, Rats, Rats, Sprague-Dawley, Receptors, Opioid, RNA, Messenger
Subjects:B Subjects allied to Medicine > B210 Pharmacology
B Subjects allied to Medicine > B140 Neuroscience
Divisions:College of Science > School of Pharmacy
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ID Code:22166
Deposited On:24 Feb 2016 14:18

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