The Mutagenesis Assistant Program

Verma, Rajni, Wong, Tuck Seng, Schwaneberg, Ulrich and Roccatano, Danilo (2014) The Mutagenesis Assistant Program. In: Directed Evolution Library Creation. Methods in Molecular Biology, 1179 (1179). Springer, pp. 279-290. ISBN 9781493910533, 9781493910526

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Mutagenesis Assistant Program (MAP) is a web-based statistical tool to develop directed evolution strategies by investigating the consequences at the amino acid level of the mutational biases of random mutagenesis methods on any given gene. The latest development of the program, the MAP2.03D server, correlates the generated amino acid substitution patterns of a specific random mutagenesis method to the sequence and structural information of the target protein. The combined information can be used to select an experimental strategy that improves the chances of obtaining functionally efficient and/or stable enzyme variants. Hence, the MAP2.03D server facilitates the “in silico” prescreening of the target gene by predicting the amino acid diversity generated in a random mutagenesis library. Here, we describe the features of MAP2.03D server by analyzing, as an example, the cytochrome P450BM3 monooxygenase (CYP102A1). The MAP2.03D server is available publicly at http://​map.​jacobs-university.​de/​map3d.​html.

Keywords:Directed evolution, Random mutagenesis methods, Mutational bias, Residue mutability indicator, protein engineering
Subjects:C Biological Sciences > C700 Molecular Biology, Biophysics and Biochemistry
Divisions:College of Science > School of Mathematics and Physics
ID Code:19395
Deposited On:27 Oct 2015 15:20

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