Insulin release from human pancreatic islets in vitro

Grant, A. M., Christie, M. R. and Ashcroft, S. J. H. (1980) Insulin release from human pancreatic islets in vitro. Diabetologia, 19 (2). pp. 114-117. ISSN 0012-186X

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Islets of Langerhans were isolated by collagenase digestion from the pancreas of a 39 year-old female renal transplant donor. The islets were subjected to three consecutive periods of tissue culture, after each of which they were incubated in vitro with various agents whose effects on insulin release from islets of laboratory animals have previously been established. After the first culture period, the basal insulin secretion rate of 5.2 μU/islet/h seen with 2 mmol/l glucose was increased approx. 5-fold on raising the glucose concentration to 20 mmol/l. The islets retained the insulin-secretory response to 20 mmol/l glucose throughout the period of study. Insulin secretion was also stimulated by mannose, leucine, α-ketoisocaproate, dihydroxyacetone and 3-hydroxybutyrate, but not by fructose or N-acetyl-glucosamine. Fructose however increased insulin release in the presence of 4 mmol/l glucose. Caffeine elicited insulin release in the absence of glucose and enhanced insulin release in response to 10 mmol/l glucose. Glucose-stimulated insulin release was inhibited by trifluoperazine (25 μmol/l). © 1980 Springer-Verlag.

Keywords:2 oxoisocaproic acid, 3 hydroxybutyric acid, caffeine, dihydroxyacetone, fructose, glucose, insulin, leucine, mannose, n acetylglucosamine, trifluoperazine, cell culture, endocrine system, human, human cell, in vitro study, pancreas islet, pancreas islet beta cell, tissue culture, Adult, Cells, Cultured, Female, Islets of Langerhans, Monosaccharides, Support, Non-U.S. Gov't, Trifluoperazine
Subjects:B Subjects allied to Medicine > B120 Physiology
Divisions:College of Science > School of Life Sciences
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ID Code:18180
Deposited On:31 Jul 2015 08:59

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