Optical detection of photo-activated protein adsorption using Brewster angle microscopy

Li, Y., Song, H. Y., Lear, M. J. , Gorelik, S., Tomczak, N. and Hobley, J. (2008) Optical detection of photo-activated protein adsorption using Brewster angle microscopy. In: PhotonicsGlobal@Singapore, 2008. IPGC 2008, 8-11 Dec 2008, Singapore.

Full content URL: http://dx.doi.org/10.1109/IPGC.2008.4781381


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Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266 nm light yields the pi-pi* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with the modified quartz. The triplet state may form a ketyl radical by reaction with the solution or it may abstract a proton directly from the BSA becoming covalently tethered to the surface. The entire deposition process was monitored in real time, in-situ using a Brewster angle microscope (BAM).

Keywords:Immobilisation chemistry, Brewster angle microscopy
Subjects:F Physical Sciences > F170 Physical Chemistry
F Physical Sciences > F160 Organic Chemistry
Divisions:College of Science > School of Chemistry
ID Code:17199
Deposited On:23 Apr 2015 13:40

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