Qri2/Nse4, a component of the essential Smc5/6 DNA repair complex: Nse4, an essential cell cycle protein

Hu, Bin, Liao, Chunyan, Millson, Stefan H. , Mollapour, Mehdi, Prodromou, Chrisostomos, Pearl, Laurence H., Piper, Peter W. and Panaretou, Barry (2005) Qri2/Nse4, a component of the essential Smc5/6 DNA repair complex: Nse4, an essential cell cycle protein. Molecular Microbiology, 55 (6). pp. 1735-1750. ISSN 0950-382X

Full content URL: http://dx.doi.org/10.1111/j.1365-2958.2005.04531.x

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Item Type:Article
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We demonstrate a role for Qri2 in the essential DNA repair function of the Smc5/6 complex in Saccharomyces cerevisiae. We generated temperature-sensitive (ts) mutants in QRI2 and characterized their properties. The mutants arrest after S phase and prior to mitosis. Furthermore, the arrest is dependant on the Rad24 checkpoint, and is also accompanied by phosphorylation of the Rad53 checkpoint effector kinase. The mutants also display genome instability and are sensitive to agents that damage DNA. Two-hybrid screens reveal a physical interaction between Qri2 and proteins that are non-Smc elements of the Smc5/6 DNA repair complex, which is why we propose the name NSE4 for the open reading frame previously known as QRI2. A key role for Nse4 in Smc5/6 function is likely, as overexpressing known subunits of the Smc5/6 complex suppresses nse4ts cell cycle arrest. The nse4ts growth arrest is non-lethal and unlike the catastrophic nuclear fragmentation phenotype of smc6ts mutants, the nucleus remains intact; replicative intermediates and sheared DNA are not detected. This could imply a role for Nse4 in maintenance of higher order chromosome structure.

Additional Information:First published online Feb 2005
Subjects:C Biological Sciences > C700 Molecular Biology, Biophysics and Biochemistry
C Biological Sciences > C500 Microbiology
Divisions:College of Science > School of Life Sciences
ID Code:15330
Deposited On:07 Oct 2014 19:54

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