Expression of the calcium-sensing receptor on human antral gastrin cells in culture

Ray, J. M., Squires, Paul E., Curtis, S. B. , Meloche, M. R. and Buchan, A. M. J. (1997) Expression of the calcium-sensing receptor on human antral gastrin cells in culture. Journal of Clinical Investigation, 99 (10). pp. 2328-2333. ISSN 0021-9738

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The presence of the extracellular calcium-sensing receptor on human antral gastrin cells was investigated. Reverse transcription PCR using mRNA isolated from gastrin cell-enriched cell cultures identified a product with a sequence identical to part of the human parathyroid-secreting cell calcium- sensing receptor. Immunocytochemistry with an antibody to the extracellular region of the receptor immunostained all gastrin cells (but not mucin or somatostatin cells), and detected appropriate-sized bands in Western blots of whole cell lysates. Increasing extracellular calcium levels from 0.5 to 9 mM stimulated gastrin release in a concentration-dependent manner, with maximal release obtained at 7.2 mM. A known agonist of the calcium receptor, spermine also stimulated gastrin release. Microfluorimetry of identified gastrin cells demonstrated that increasing extracellular calcium resulted in an initial rapid rise in intracellular calcium followed by a plateau level that returned to basal levels immediately after removal of the elevated calcium. The traces were consistent with activation of a receptor-mediated mechanism rather than a concentration-dependent influx of calcium. In conclusion, these data indicate that G cells express the calcium-sensing receptor, and that activation of the receptor may explain the acid rebound phenomenon associated with calcium-containing antacid preparations.

Keywords:antacid agent, calcium, calcium chloride, cell receptor, gastrin, gastrin 17, messenger rna, monoclonal antibody, mucin, silane derivative, somatostatin, somatostatin 14, spermine, adult, article, calcium transport, controlled study, female, gastrin cell, gastrin release, gene expression, human, human cell, immunocytochemistry, male, microfluorometry, parathyroid cell, priority journal, rebound, reverse transcription polymerase chain reaction, sequence homology, stomach antrum, Calcium, Calcium-Binding Proteins, Cells, Cultured, Gastric Mucosa, Gastrins, Humans, Immunohistochemistry, Kinetics, Polymerase Chain Reaction, Pyloric Antrum, RNA, Messenger, Spermine, Transcription, Genetic
Subjects:C Biological Sciences > C990 Biological Sciences not elsewhere classified
Divisions:College of Science > School of Life Sciences
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ID Code:14358
Deposited On:20 Jun 2014 11:17

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