Signaling through the p38 and p42/44 mitogen-activated families of protein kinases in pancreatic β-cell proliferation

Burns, C. J., Squires, Paul and Persaud, S. J. (2000) Signaling through the p38 and p42/44 mitogen-activated families of protein kinases in pancreatic β-cell proliferation. Biochemical and Biophysical Research Communications, 268 (2). pp. 541-546. ISSN 0006-291X

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The present study has focused on the role of the 42- and 44-kDa mitogen-activated protein kinases (p42/44 MAPKs) and the 38-kDa mitogen-activated protein kinase (p38 MAPK) in the proliferation of the pancreatic β-cell line MIN6. MIN6 β-cell proliferation was assessed by measuring 5-bromo-2'-deoxyuridine (BrdU) incorporation into cellular DNA. Inhibition of both the p42/44 MAPK pathway using the MEK inhibitor PD098059 (PD) and the p38 MAPK pathway using the p38 inhibitor SB203580 (SB) caused a marked, concentration-dependent reduction in the BrdU immunostaining observed in the presence of 15 FCS when assessed using fluorescence immunocytochemistry. These data provide direct evidence of a role for p42/44 MAPKs in the mitogenic response of MIN6 β-cells to FCS. Furthermore, these data also suggest a novel role for the p38 MAPK pathway in MIN6 β-cell proliferation. (C) 2000 Academic Press.

Keywords:2 (2 amino 3 methoxyphenyl)chromone, 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole, broxuridine, mitogen activated protein kinase, protein kinase, article, cell proliferation, controlled study, human, human cell, mitogenesis, pancreas islet beta cell, priority journal, Animals, Ca(2+)-Calmodulin Dependent Protein Kinase, Cell Division, Enzyme Inhibitors, Flavonoids, Humans, Imidazoles, Islets of Langerhans, MAP Kinase Signaling System, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Mitogen-Activated Protein Kinases, p38 Mitogen-Activated Protein Kinases, Pyridines, Rats
Subjects:C Biological Sciences > C990 Biological Sciences not elsewhere classified
Divisions:College of Science > School of Life Sciences
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ID Code:14352
Deposited On:18 Jun 2014 14:33

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