Role of adenine nucleotides in insulin secretion from MIN6 pseudoislets

Hauge-Evans, A. C., Squires, Paul E., Belin, V. D. , Roderigo-Milne, H., Ramracheya, R. D., Persaud, S. J. and Jones, P. M. (2002) Role of adenine nucleotides in insulin secretion from MIN6 pseudoislets. Molecular and Cellular Endocrinology, 191 (2). pp. 167-176. ISSN 0303-7207

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Insulin secretion from MIN6 cells configured as cell aggregates by culture on a gelatin substrate (pseudoislets) is enhanced compared to that of MIN6 cells grown as monolayers on tissue culture plastic, indicating the importance of β-cell-to-β-cell proximity for insulin release. In this study we have shown that glucose induced a biphasic release of insulin from pseudoislets, whereas the amplitude and duration of the responses of equivalent monolayer cells were much reduced. Purinergic aqonists have been implicated in intercellular communication between β-cells, so we investigated whether adenine nucleotides co-released with insulin are responsible for the enhanced secretory responses of pseudoislets. We have demonstrated that MIN6 cells express purinergic A1 and P2Y receptors, and that adenine nucleotides increased [Ca2+] with an efficacy of agonists being ATP>ADP>AMP. However, neither suramin nor the more selective A1 antagonist 1,3-dipropyl-8-cyclopentylxanthine reduced glucose-induced insulin secretion from pseudoislets, and stimulation of monolayer cells with a range of adenine nucleotides did not enhance glucose-induced secretion. These results suggest that enhanced secretion from MIN6 pseudoislets is not due to increased paracrine/autocrine action of adenine nucleotides. © 2002 Elsevier Science Ireland Ltd. All rights reserved.

Keywords:8 cyclopentyl 1,3 dipropylxanthine, adenine nucleotide, adenosine diphosphate, adenosine phosphate, adenosine triphosphate, calcium ion, glucose, insulin, purine a1 receptor, purine P2Y receptor, purine receptor, suramin, unclassified drug, uridine triphosphate, animal tissue, article, autocrine effect, cell aggregation, cell communication, cell stimulation, controlled study, immunoreactivity, insulin release, monolayer culture, mouse, nonhuman, pancreas islet beta cell, priority journal, protein expression, Adenine Nucleotides, Animals, Autocrine Communication, Calcium, Cell Line, Glucokinase, Glucose Transporter Type 2, Islets of Langerhans, Kinetics, Mice, Monosaccharide Transport Proteins, Paracrine Communication
Subjects:C Biological Sciences > C990 Biological Sciences not elsewhere classified
Divisions:College of Science > School of Life Sciences
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ID Code:14327
Deposited On:13 Jun 2014 08:57

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