Expression and function of the extracellular calcium-sensing receptor in pancreatic beta-cells

Jones, Peter M., Kitsou-Mylona, Isidora, Gray, Elizabeth , Squires, Paul E. and Persaud, Shanta J. (2007) Expression and function of the extracellular calcium-sensing receptor in pancreatic beta-cells. Archives of physiology and biochemistry, 113 (3). pp. 98-103. ISSN 1381-3455

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The extracellular calcium-sensing receptor (CaR) was first identified in tissues involved in systemic Ca2+ homeostasis, where it acts to sense changes in circulating Ca2+. It has since been reported that the CaR is expressed in many tissues that are not associated with Ca2+ homeostasis, including the endocrine cells in pancreatic islets of Langerhans. In the present study we have used an insulin-secreting pancreatic beta-cell line (MIN6) to investigate the expression and function of CaR, using the calcimimetic A568, a CaR agonist that activates the CaR at physiological concentrations of extracellular Ca2+ ([Ca2+]o). Immunocytochemistry, Western blotting and RT-PCR confirmed the expression of CaR in MIN6 cells. CaR activation was associated with rapid and transient increases in [Ca2+]o, which were accompanied by the initiation of a marked but transient insulin secretory response. Stimulation of beta-cell secretory activity had no detectable effect on CaR mRNA levels, but CaR mRNA was markedly reduced by configuring MIN6 cells into islet- like structures. Our data are consistent with an important function for the beta-cell CaR in cell - cell communication within islets to co-ordinate insulin secretory responses.

Keywords:Cyclooxygenases, Lipoxygenases, Islands of Langerhans, Insulin, Arachidonic acid, Prostaglandins, Pharmaceutical and medicine manufacturing, Medicinal and botanical manufacturing
Subjects:C Biological Sciences > C720 Biological Chemistry
Divisions:College of Science > School of Life Sciences
ID Code:14240
Deposited On:05 Jun 2014 10:05

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