ANP stimulates hepatocyte Ca2+ efflux via plasma membrane recruitment of PKGIα

Stratton, Rebecca C., Squires, Paul E. and Green, Anne K. (2008) ANP stimulates hepatocyte Ca2+ efflux via plasma membrane recruitment of PKGIα. Biochemical and Biophysical Research Communications, 36 (4). pp. 965-970. ISSN 0006-291X

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In rat hepatocytes, atrial natriuretic peptide (ANP) elevates cGMP through activation of particulate guanylyl cyclase and attenuates Ca2+ signals by stimulating net plasma membrane Ca2+ efflux. We show here that ANP-stimulated hepatocyte Ca2+ efflux is mediated by protein kinase G (PKG) isotype I. Furthermore, we show that ANP recruits endogenous PKGIα, but not PKGIβ, to the plasma membrane. These effects are mimicked by 8-bromo-cGMP, but not by the soluble guanylyl cyclase activators, sodium nitroprusside and YC-1. We propose that ANP, through localized cGMP elevation, promotes plasma membrane recruitment of PKGIα, which, in turn, stimulates Ca2+ efflux.

Keywords:Atrial natriuretic peptide (ANP), Ca2+ efflux, cGMP compartmentation, cGMP-dependent protein kinase (cGK), Plasma membrane Ca2+-ATPase (PMCA), Protein kinase G (PKG), Hepatocytes
Subjects:Library of Congress Subject Areas > Q Science > QH Natural history > QH301 Biology
Library of Congress Subject Areas > Q Science > QP Physiology
Divisions:College of Science > School of Life Sciences
ID Code:14076
Deposited On:30 Aug 2011 13:12

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