Lipid-dependent GPCR dimerization

Goddard, Alan D., Dijkman, Patricia M., Adamson, Roslin J. and Watts, Anthony (2013) Lipid-dependent GPCR dimerization. Methods in Cell Biology, 117 . pp. 341-357. ISSN 0091-679X

Full content URL:

Full text not available from this repository.

Item Type:Article
Item Status:Live Archive


It has been widely demonstrated that G protein-coupled receptors (GPCRs) can form dimers both in vivo and in vitro, a process that has functional consequences. These receptor-receptor interactions take place within a phospholipid bilayer, yet, generally, little is known of the requirements for specific lipids that mediate the dimerization process. Studying this phenomenon in vivo is challenging due to difficulties in modulating the lipid content of cell membranes. Therefore, in this chapter, we describe techniques for reconstitution of GPCRs into model lipid bilayers of defined composition. The concentrations of specific lipids and sterols can be precisely controlled in these liposomes, as well as maintaining an appropriate lipid-protein ratio to avoid artifactual interactions. Receptor dimerization in this system is monitored via Förster resonance energy transfer (FRET), which requires the use of fluorescently labeled receptors. We therefore also include protocols for labeling with appropriate fluorophores and determining the apparent FRET efficiency, a measurement of the extent of receptor dimerization. Understanding the lipid dependence of GPCR dimerization will be key in understanding how this process is regulated in the dynamic heterogeneous environment of the cell membrane.

Keywords:Dimer, FRET, GPCR
Subjects:C Biological Sciences > C700 Molecular Biology, Biophysics and Biochemistry
Divisions:College of Science > School of Life Sciences
ID Code:12914
Deposited On:09 Jan 2014 14:08

Repository Staff Only: item control page