Molecular cloning and characterization of buffalo αs1-casein gene

Sukla, S., Bhattacharya, T. K., Venkatachalapathy, R. T. , Kumar, P. and Sharma, A. (2007) Molecular cloning and characterization of buffalo αs1-casein gene. Mitochondrial DNA, 18 (5). pp. 334-340. ISSN 1940-1736

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Buffaloes in Indian subcontinent play an important role as the producer of milk and milk products. The αs1-casein constitutes 38% of the total milk proteins. The present study was carried out to characterize the gene in Murrah breed of Riverine buffalo. Buffalo αs1-casein cDNA was synthesized by RT-PCR, then cloned using pDRIVE-cloning vector and sequenced. The sequencing revealed that the size of αs1-casein cDNA was of 645 bp with GC content of 45.58%. The αs1-casein gene coded 214 amino acids precursor with a signal peptide of 15 amino acid residues. The similarity of buffalo αs1-casein mRNA sequence with that of cattle, goat, sheep, pig, camel, equine and human were estimated as 97.2, 93, 92.3, 57.2, 59.5, 55.9 and 46.6%, respectively. A similar trend was observed when compared amino acid sequences of these species. In the phylogenetic trees, constructed from the data of the αs1-casein mRNA as well as protein sequences, it has been observed that buffalo, cattle, goat and sheep formed a cluster with a closer relationship between cattle and buffalo followed by goat and sheep.

Keywords:Buffalo, ?s1-casein, Cloning, Sequencing, Phylogenetic tree, DQ111783
Subjects:C Biological Sciences > C432 Veterinary Genetics
Divisions:College of Science > School of Life Sciences
ID Code:12912
Deposited On:10 Jan 2014 08:47

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