Interfacial study of cell adhesion to liquid crystals using widefield surface plasmon resonance microscopy

Soon, Chin Phong, Khaghani, Seyed Ali, Youseffi, Mansour , Nayan, Nafarizal, Saim, Hashim, Britland, Stephen, Blagden, Nicholas and Denyer, Morgan Clive Thomas (2013) Interfacial study of cell adhesion to liquid crystals using widefield surface plasmon resonance microscopy. Colloids and Surfaces B: Biointerfaces, 110 . pp. 156-162. ISSN 0927-7765

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Widefield surface plasmon resonance (WSPR) microscopy provides high resolution imaging of interfacial interactions. We report the application of the WSPR imaging system in the study of the interaction between keratinocytes and liquid crystals (LC). Imaging of fixed keratinocytes cultured on gold coated surface plasmon substrates functionalized with a thin film of liquid crystals was performed in air using a 1.45. NA objective based system. Focal adhesion of the cells adhered to glass and LC were further studied using immunofluorescence staining of the vinculin. The imaging system was also simulated with 2. � 2 scattering matrix to investigate the optical reflection of the resonant plasmonic wave via the glass/gold/cell and glass/gold/LC/cell layers. WSPR imaging indicated that keratinocytes are less spread and formed distinct topography of cell-liquid crystal couplings when cultured on liquid crystal coated substrates. The simulation indicates that glass/LC shifted the surface plasmon excitation angle to 75.39° as compared to glass/air interface at 44°. The WSPR microcopy reveals that the cells remodelled their topography of adhesion at different interfaces. © 2013 Elsevier B.V.

Keywords:Crystal interfaces, High-resolution imaging, Immunofluorescence staining, Interfacial interaction, Keratinocytes, Scattering matrices, Surface plasmon excitation, Wide-field, Cell adhesion, Cells, Glass, Image reconstruction, Imaging systems, Liquid crystals, Scattering parameters, Surface plasmon resonance, Plasmons, vinculin, article, cell adhesion, cell interaction, cell surface, cellular distribution, chemical phenomena, computer simulation, controlled study, culture technique, human, human tissue, imaging system, immunocytochemistry, immunofluorescence, keratinocyte, liquid crystal, material coating, microscopy, molecular interaction, optical density, optical reflection, priority journal, refraction index, surface property, widefield surface plasmon resonance microscopy
Subjects:B Subjects allied to Medicine > B200 Pharmacology, Toxicology and Pharmacy
Divisions:College of Science > School of Pharmacy
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ID Code:11363
Deposited On:30 Jul 2013 14:43

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