Singh, Ishwar, Freeman, Colin and Heaney, Frances (2011) Efficient synthesis of DNA conjugates by strain-promoted azide-cyclooctyne cycloaddition in the solid phase. European Journal of Organic Chemistry (33). pp. 6739-6746. ISSN 1434-193X
Full content URL: http://dx.doi.org/10.1002/ejoc.201101045
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Item Type: | Article |
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Item Status: | Live Archive |
Abstract
Conjugation of ligands to DNA oligonucleotides has been achieved in the solid phase by strain-promoted azide-alkyne cycloaddition (SPAAC). The oligonucleotide, modified with a simple nonfluroinated, monocyclic octyne, efficiently forms conjugates with a range of azide dipoles with varying steric and electronic characteristics. The reaction is clean and easily executed in a copper free environment at room temperature. It provides a variety of triazole-linked nucleic acid conjugates and is potentially useful in biotechnology and cell biology.
Additional Information: | Article first published online: 19 SEP 2011 |
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Keywords: | Medicinal chemistr, Oligonucleotides, DNA, Solid-phase synthesis, Click reaction, Azides, Alkynes, Cycloaddition, Bioconjugation} |
Subjects: | B Subjects allied to Medicine > B200 Pharmacology, Toxicology and Pharmacy |
Divisions: | College of Science > School of Pharmacy |
ID Code: | 11292 |
Deposited On: | 25 Jul 2013 15:33 |
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