Sequence analysis of Vpg protein of Asia-1 field isolates of Foot and Mouth Disease Virus in India

Biswas, S., Sanyal, A., Hemadri, D., Tosh, C., Gupta, S. K., Mittal, M., Mohapatra, J. K., Sabarinath, G. P., Kumar, R. Manoj and Bandyopadhyay, S. K. (2004) Sequence analysis of Vpg protein of Asia-1 field isolates of Foot and Mouth Disease Virus in India. Journal of Animal and Veterinary Advances, 3 (8). pp. 522-526. ISSN 1680-5593

Full content URL: http://medwelljournals.com/abstract/?doi=javaa.200...

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Item Type:Article
Item Status:Live Archive

Abstract

Foot-and-Mouth Disease (FMD), the most economically important disease of cloven-hoofed animals is endemic in India. A total of twenty field isolates belonging to serotype Asia1, collected from different states in India during 1986-2002 were taken as BHK-21 cell culture adapted virus from the National Repository of FMDV at the Project Directorate on Foot-and-Mouth Disease (PD FMD). RT-PCR products of the revived viruses were confirmed by agarose gel electrophoresis and the gel-purified products were subjected to cycle sequencing in an automated sequencer. Vpg protein, which on uridylation serves as a primer during FMDV replication is found conserved across serotypes O, A and C. The sequences generated in the present work were compared with respect to other serotypes with the objective of investigating the amino acid variability of the three redundant copies of VPg proteins in a diverse group field isolates of type Asia1.

Keywords:RNA virus, Virus evolution, molecular phylogeny
Subjects:C Biological Sciences > C540 Virology
C Biological Sciences > C520 Medical and Veterinary Microbiology
Divisions:College of Science > School of Life Sciences
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ID Code:9380
Deposited On:10 May 2013 08:21

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