Wavefront engineering microscopy to study 3D mechanotransduction in living cells

Emiliani, Valentina, Cojoc, Dan, Ferrari, Enrico, Garbin, Valeria, Durieux, Christiane and Di Fabrizio, Enzo (2006) Wavefront engineering microscopy to study 3D mechanotransduction in living cells. In: Nanophotonics, 3-5 April 2006, Strasbourg, France.

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Wavefront engineering microscopy to study 3D mechanotransduction in living cells

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Abstract

We present an experimental system based on the use of a spatial light modulator which enables to perform simultaneously 3D optical manipulation and optical sectioning. This has been achieved by modifying the wave front of the trapping beam with properly diffractive optical elements displayed on a computer controlled spatial light modulator. We demonstrated the capability of the system in two experimental schemes, in a first one we performed a 3D optical scanning of 6 trapped beads by displacing the beads through a fixed imaging plane. In a second one we scan the imaging plane and simultaneously compensate for the movement of the objective in order to keep the trapping plane at a fixed position.

Additional Information:Nanophotonics 2006
Keywords:Diffractive optical elements
Subjects:F Physical Sciences > F310 Applied Physics
Divisions:College of Science > School of Life Sciences
ID Code:8398
Deposited On:27 Mar 2013 10:39

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