Lappin, Graham, Simpson, M., Shishikura, Y. and Garner, C. (2008) High-performance liquid chromatography accelerator mass spectrometry: Correcting for losses during analysis by internal standardization. Analytical Biochemistry, 378 (1). pp. 93-95. ISSN 0003-2697
Full content URL: http://dx.doi.org/10.1016/j.ab.2008.03.026
Full text not available from this repository.
Item Type: | Article |
---|---|
Item Status: | Live Archive |
Abstract
A method was developed to account for analytical losses of 14C-analyte when determining the concentration in biological samples using chromatographic separation and analysis by accelerator mass spectrometry. From the equations of J. Vogel and A.H. Love (in: A.L. Burlingame (Ed.), Methods in Enzymology, Academic Press, New York, 2005), new equations were derived to describe the isotopic dilution of a chromatographically isolated 14C-analyte. The analytical recovery for each sample was determined by the use of the UV response for nonlabeled analyte, as an internal standard against a standard curve. The slope of the curve was substituted into the equations to provide a method of accurately determining the analyte concentration. © 2008 Elsevier Inc. All rights reserved.
Keywords: | carbon 14, accelerator mass spectrometry, analytic method, article, diagnostic accuracy, dilution, high performance liquid chromatography, mass spectrometry, mathematical computing, priority journal, separation technique, standardization, ultraviolet radiation, Chromatography, High Pressure Liquid |
---|---|
Divisions: | College of Science > School of Pharmacy |
Related URLs: | |
ID Code: | 8226 |
Deposited On: | 24 Mar 2013 21:16 |
Repository Staff Only: item control page