Novel approach for effective isolation of mesenchymal stem cells from human umbilical cord blood

Hussain, Issam, Maged, Salah, Sloan, Andrew , Eremin, Oleg and El-Sheemy, Mohamed (2009) Novel approach for effective isolation of mesenchymal stem cells from human umbilical cord blood. In: European Society for Surgical Research (ESSR); 44th Congress, 19-24 May 2009, Nimes, France.

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Item Type:Conference or Workshop contribution (Presentation)
Item Status:Live Archive

Abstract

Human umbilical cord blood (HUCB) has now been successfully used in clinical allogenic transplantation as an efficacious supply of haematopoietic stem cells (HSC). However, HUCB has been poorly recognised as a good source for Mesenchymal stem cells (MSCs). The aims of this study, therefore, are to define and characterize the content of HUCB as a rich source for MSCs using a new method. .
Method: Voluntary consented candidates, who underwent elective caesarean section, HUCB was collected (n=9), explanted and cultured in Mesencult basal media and maintained at pH 7.3 in a humidified incubator at 37°C with 5% CO2. Immunophenotyping qualitative technique using fluorescein isothiocyanate (FITC) was applied to the cells, and the surface antigen markers CD34, CD29, CD45, CD73 and CD105 were utilized. MSCs were then plated in Neurogenic and Adipogenic differentiated media. Immunocytochemistry was used for identification of SOX2, Olig4 and FABP-4.
Result: MSCs went through morphological changes to become spindle shaped with potential to become part of a developmental cell lineage. Cells were negative for HSC antigen markers CD34 and CD45, but strongly positive for MSCs markers CD29, CD44, CD73 and CD105. MSCs expressed neural markers SOX2, Olig4 and the adipogenic marker FABP-4 with lipid vacuoles also observed.
Conclusion: HUCB is rich source of MSCs using this novel technique.

Additional Information:Human umbilical cord blood (HUCB) has now been successfully used in clinical allogenic transplantation as an efficacious supply of haematopoietic stem cells (HSC). However, HUCB has been poorly recognised as a good source for Mesenchymal stem cells (MSCs). The aims of this study, therefore, are to define and characterize the content of HUCB as a rich source for MSCs using a new method. . Method: Voluntary consented candidates, who underwent elective caesarean section, HUCB was collected (n=9), explanted and cultured in Mesencult basal media and maintained at pH 7.3 in a humidified incubator at 37°C with 5% CO2. Immunophenotyping qualitative technique using fluorescein isothiocyanate (FITC) was applied to the cells, and the surface antigen markers CD34, CD29, CD45, CD73 and CD105 were utilized. MSCs were then plated in Neurogenic and Adipogenic differentiated media. Immunocytochemistry was used for identification of SOX2, Olig4 and FABP-4. Result: MSCs went through morphological changes to become spindle shaped with potential to become part of a developmental cell lineage. Cells were negative for HSC antigen markers CD34 and CD45, but strongly positive for MSCs markers CD29, CD44, CD73 and CD105. MSCs expressed neural markers SOX2, Olig4 and the adipogenic marker FABP-4 with lipid vacuoles also observed. Conclusion: HUCB is rich source of MSCs using this novel technique.
Keywords:Human Umbilical Cord Blood, Mesenchymal stem cells, Novel method, bmjtype, bmjlink
Subjects:C Biological Sciences > C130 Cell Biology
Divisions:College of Science > School of Life Sciences
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ID Code:7473
Deposited On:09 Feb 2013 11:03

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