Examining Local Cell-to-Cell Signalling in the Kidney Using ATP Biosensing

Price, Gareth, Potter, Joe, Williams, Bethany , Cliff, Chelsy, Wall, Mark, Hills, Claire and Squires, Paul (2020) Examining Local Cell-to-Cell Signalling in the Kidney Using ATP Biosensing. In: Methods in Molecular Biology. Springer, New York. ISBN UNSPECIFIED

Full content URL: https://doi.org/10.1007/7651_2020_297

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Examining Local Cell-to-Cell Signalling in the Kidney Using ATP Biosensing

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Abstract

Cell-to-cell communication is an essential process for the efficient function of cells and tissues. Central to this is the purinergic transmission of purines, with ligands such as adenosine triphosphate (ATP). Altered cell-to-cell communication, and in particular changes in the paracrine release of extracellular ATP, plays crucial roles in pathophysiological conditions, such as diabetes. ATP biosensing provides a reliable, real-time measurement of local extracellular ATP concentrations. This allows the detection of altered ATP release, which underlies the progression of inflammation and fibrosis and is a potential therapeutic target. Here we describe in a step-by-step basis how to utilize sensitive microelectrode biosensors to detect low, real-time concentrations of ATP, in vitro.

Additional Information:On line ahead of print
Keywords:Adenosine triphosphate, Adhesion, Biosensing, connexins, purines, Cell-to-cell communication
Subjects:C Biological Sciences > C130 Cell Biology
B Subjects allied to Medicine > B120 Physiology
C Biological Sciences > C100 Biology
Divisions:College of Science > School of Life Sciences
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ID Code:41509
Deposited On:17 Jul 2020 09:58

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