UCHL3 Regulates Topoisomerase-Induced Chromosomal Break Repair by Controlling TDP1 Proteostasis

Liao, Chunyan, Beveridge, Ryan, Hudson, Jessica J.R. R , Parker, Jacob D., Chiang, Shih-Chieh Chieh, Ray, Swagat, Ashour, Mohamed E., Sudbery, Ian, Dickman, Mark J. and El-Khamisy, Sherif F. (2018) UCHL3 Regulates Topoisomerase-Induced Chromosomal Break Repair by Controlling TDP1 Proteostasis. Cell Reports, 23 (11). pp. 3352-3365. ISSN 22111247

Full content URL: https://linkinghub.elsevier.com/retrieve/pii/S2211...

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Item Type:Article
Item Status:Live Archive

Abstract

Genomic damage can feature DNA-protein crosslinks whereby their acute accumulation is utilized to treat cancer and progressive accumulation causes neurodegeneration. This is typified by tyrosyl DNA phosphodiesterase 1 (TDP1), which repairs topoisomerase-mediated chromosomal breaks. Although TDP1 levels vary in multiple clinical settings, the mechanism underpinning this variation is unknown. We reveal that TDP1 is controlled by ubiquitylation and identify UCHL3 as the deubiquitylase that controls TDP1 proteostasis. Depletion of UCHL3 increases TDP1 ubiquitylation and turnover rate and sensitizes cells to TOP1 poisons. Overexpression of UCHL3, but not a catalytically inactive mutant, suppresses TDP1 ubiquitylation and turnover rate. TDP1 overexpression in the topoisomerase therapy-resistant rhabdomyosarcoma is driven by UCHL3 overexpression. In contrast, UCHL3 is downregulated in spinocerebellar ataxia with axonal neuropathy (SCAN1), causing elevated levels of TDP1 ubiquitylation and faster turnover rate. These data establish UCHL3 as a regulator of TDP1 proteostasis and, consequently, a fine-tuner of protein-linked DNA break repair. Deciphering the mechanisms regulating protein homeostasis is clinically important. Liao et al. identify UCHL3 as a key player in ubiquitylation and turnover of the DNA repair enzyme TDP1. Disruption of TDP1's ubiquitylation status causes neurological disease or resistance to topoisomerase I-targeting chemotherapy in cancer.

Keywords:SCAN1,TDP,UCHL3,aging,cancer,heart failure,myocardial infarction,neurodegeneration,rhabdosarcoma,topoisomerase
Subjects:C Biological Sciences > C130 Cell Biology
Divisions:College of Science > School of Life Sciences
ID Code:37141
Deposited On:27 Sep 2019 10:17

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