Effect of an electrolyte cation on detecting DNA damage with the latch onstriction of α-hemolysin

Johnson, Robert P., Fleming, Aaron M., Burrows, Cynthia J. and White, Henry S. (2014) Effect of an electrolyte cation on detecting DNA damage with the latch onstriction of α-hemolysin. The Journal of Physical Chemistry Letters, 5 (21). pp. 3781-3786. ISSN 1948-7185

Documents
Effect of an Electrolyte Cation on Detecting DNA Damage with the Latch Constriction of α-Hemolysin.pdf

Request a copy
[img] PDF
Effect of an Electrolyte Cation on Detecting DNA Damage with the Latch Constriction of α-Hemolysin.pdf - Whole Document
Restricted to Repository staff only

1MB
Item Type:Article
Item Status:Live Archive

Abstract

The effect of an electrolyte cation on the unzipping of furan-containing double-stranded DNA in an α-hemolysin (αHL) nanopore is described. The current through an open αHL channel increases in proportion to the ion mobility. However, the ionic current measured during residence of a DNA duplex inside of the protein pore shows a more complex dependence on the choice of cation, indicating that the current measured during DNA residence in the pore is modulated by the specific interactions of the cations with the DNA and/or αHL. The residence time (stability) of the DNA duplex inside of the pore prior to unzipping is also highly dependent on the cation, in striking contrast to the small variation in duplex stability (as measured by the melting temperature) in bulk electrolyte solution. A missing base in DNA can be detected in the latch region of αHL with optimal current resolution in RbCl, while optimal time resolution is possible in LiCl.

Keywords:DNA Damage, Hemolysin, Nanopores, NotOAChecked
Subjects:F Physical Sciences > F170 Physical Chemistry
Divisions:College of Science > School of Chemistry
Related URLs:
ID Code:27414
Deposited On:17 May 2017 15:06

Repository Staff Only: item control page