A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations

Johnson, Robert P., Richardson, James A., Brown, Tom and Bartlett, Philip N. (2012) A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations. Journal of the American Chemical Society, 134 (34). pp. 14099-14107. ISSN 0002-7863

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Abstract

A label-free, surface-enhanced Raman spectroscopy-based assay for detecting DNA hybridization at an electrode surface and for distinguishing between mutations in DNA is demonstrated. Surface-immobilized DNA is exposed to a binding agent that is selective for dsDNA and acts as a reporter molecule. Upon application of a negative potential, the dsDNA denatures into its constituent strands, and the changes in the spectra of the reporter molecule are monitored. This method has been used to distinguish between a wild-type, 1653C/T single-point mutation and ΔF508 triplet deletion in the CFTR gene. The use of dsDNA-selective binding agents as reporter molecules in a discrimination assay removes the burden of synthetically modifying the target to be detected, while retaining flexibility in the choice of the reporter molecule.

Keywords:SERS, DNA, Raman Spectroscopy, Electrochemical Melting
Subjects:F Physical Sciences > F170 Physical Chemistry
Divisions:College of Science > School of Chemistry
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ID Code:27409
Deposited On:17 May 2017 13:59

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